- Geology & Geological
- Prospective Students
- Current Students
Laser Scanning Microscopy
The laser scanning microscope utilized by AEM is an LSM 5 Pascal produced by the German manufacturer Carl Zeiss AG. The LSM 5 Pascal is a confocal microscope system that scans a specimen with laser light, point-by-point and line-by-line, in order to acquire an optical section. Many optical sections collected from different Z planes form a 3D image stack. Scanning at different levels as the laser focus is shifted along the Z axis generates a series of optical sections (slices), which can then be combined into a three-dimensional image stack. Pixel resolutions of confocal images can be freely selected between 4 x 1 and 2,048 x 2,048 pixels.
This system can be used to measure and image the topography of surfaces (This microscope is also used as an optical microscope with a halogen light). Bright field, dark field and differential interferometric contrast (DIC) are combined with features to analyze shape and size of particles, automatically measure particle and grain sizes and calculate volumetric percentages.)